Development of a visible loop mediated isothermal amplification assay for rapid detection of Bacillus anthracis

Distressing effects on animal and human health with lethal progression, being used as bioweapon shared features non-pathogenic bacteria demands sensitive, specific, safe, cost effective rapid detection methods for anthrax causing organisms. Conventional microbiology based diagnostics are time consuming need sophisticated equipment, while molecular require less labor. The Loop mediated isothermal amplification assay (LAMP) is rapid, sensitive specific requires no specialized equipment. In the present study, we developed a LAMP well of Bacillus anthracis. optimized produced positive results Sterne strain one field isolate B. anthracis and, negative other same different genera within 2 h. Sensitivity was 500 fg total DNA anthracis, which 100 times more than conventional PCR. study also demonstrated that simple method extraction by repeated boiling freezing will not adversely affect results. conclusion, promising tool time-consuming diagnosis also, detecting virulence suspected cultures.